LncRNA ACTA2-AS1 suppress colon adenocarcinoma progression by sponging miR-4428 upregulation BCL2L11

Abstract Background Long non-coding RNA is considered to be essential modulate the development and progression of human malignant cancers. And long can act as crucial modulators by sponging corresponding microRNA in tumorigenesis. We aimed elucidate function ACTA2-AS1 its molecular mechanism colon adenocarcinoma. Materials methods The expression ACTA2-AS1, miR-4428 BCL2L11 adenocarcinoma tissues were detected via qRT-PCR. SW480 HT29 cells transfected with shRNA OE miRNA mimics miR-4428, inhibitor, si- over-expression . Cell proliferation, colony formation apoptosis respectively assessed using CCK-8 assay, assay flow cytometry. Luciferase reporter was performed verify targets miR-4428. Tumor subcutaneous xenograft mode constructed explore tumor growth vivo. Results obviously downregulated cell lines. Silence or promoted inhibited proliferation abilities, regulated apoptosis. silence resulted decrease Bax increase Bal2, while restored group when compared control cells. In addition, luciferase revealed that interacted suppressed expression. could bind 3? untranslated region modulated negatively. Knockdown reversed biological mediated knockdown alone. Conclusion Our data demonstrated suppress regulate